Terminator

Part:BBa_K110002

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)


Between STE2-W and BST1-C

This terminator can be used for two genes in which the first gene (from left to right) is on the watson strand and the second is on the crick strand. It was isolated by finding an UTR between two close W-C orented genes and going 200 bp into both genes it flanked.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 417
    Illegal PstI site found at 8
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 8
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 417
    Illegal PstI site found at 8
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 417
    Illegal PstI site found at 8
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None